Goal
The objective of this catalase lab is to design simple experiments to show how different factors impact the rate of enzyme activity. This laboratory shows the way the enzyme decomposes in hydrogen peroxide. Methods and Supplies
Refer to handout attached to the spine of laboratory
Observations
Stand 1: The mL of oxygen produced with enhance of catalase
30secs
60secs
90secs
120secs
150secs
180secs
Disks: a couple of
17ml
16ml
21ml
26ml
31ml
35ml
Disks: 5
8ml
19ml
27ml
35ml
44ml
53ml
Disks: six
15ml
23ml
32ml
44ml
54ml
65ml
Disks: eight
25ml
42ml
70ml
90ml
100ml at 2: 20 secs
Disks: 10
29ml
58ml
87ml
100ml by 1: 46 secs
Desk 2: The mL of oxygen created with enhance of H202
30secs
60secs
90secs
120secs
150secs
180secs
10ml
19ml
25ml
30ml
35ml
40ml
44ml
20ml
24ml
34ml
43ml
54ml
64ml
76ml
30ml
14ml
22ml
26ml
32ml
37ml
42ml
40ml
22ml
29ml
37ml
45ml
52ml
59ml
50ml
23ml
32ml
39ml
42ml
54ml
61ml
Discussion
A catalase is a great enzyme that catalyzes the reduction of hydrogen peroxide. This enzyme is vital since it prevents the build-up of H202 in your body. This laboratory was completed to analyze and experience how H202 is usually decomposed in several concentrations of catalase and H202. It was done by raising the number of catalase in the lab with the equivalent H202, after which increasing the amount of H202 while using same amount of catalase to observe results and patterns. Graph one particular shows the increased decomposition of H202 that was produced if the amount of catalase was increased by 2 hard disk drives every time. The more catalase that was added, the more quickly the decomposition was. The fastest and the most efficient decomposition was once there were 12 catalase filtering papers in 10mL of H202. The graduated cylinder was stuffed with oxygen in 1 minute and 46 seconds. Graph 2 shows that the decomposition rate slept relatively comparable with two catalase hard disk drives and different, elevating amounts of H202. This...