09.08.2019
 Catalase Laboratory Essay

Goal

The objective of this catalase lab is to design simple experiments to show how different factors impact the rate of enzyme activity. This laboratory shows the way the enzyme decomposes in hydrogen peroxide. Methods and Supplies

Refer to handout attached to the spine of laboratory

Observations

Stand 1: The mL of oxygen produced with enhance of catalase

30secs

60secs

90secs

120secs

150secs

180secs

Disks: a couple of

17ml

16ml

21ml

26ml

31ml

35ml

Disks: 5

8ml

19ml

27ml

35ml

44ml

53ml

Disks: six

15ml

23ml

32ml

44ml

54ml

65ml

Disks: eight

25ml

42ml

70ml

90ml

100ml at 2: 20 secs

Disks: 10

29ml

58ml

87ml

100ml by 1: 46 secs

Desk 2: The mL of oxygen created with enhance of H202

30secs

60secs

90secs

120secs

150secs

180secs

10ml

19ml

25ml

30ml

35ml

40ml

44ml

20ml

24ml

34ml

43ml

54ml

64ml

76ml

30ml

14ml

22ml

26ml

32ml

37ml

42ml

40ml

22ml

29ml

37ml

45ml

52ml

59ml

50ml

23ml

32ml

39ml

42ml

54ml

61ml

Discussion

A catalase is a great enzyme that catalyzes the reduction of hydrogen peroxide. This enzyme is vital since it prevents the build-up of H202 in your body. This laboratory was completed to analyze and experience how H202 is usually decomposed in several concentrations of catalase and H202. It was done by raising the number of catalase in the lab with the equivalent H202, after which increasing the amount of H202 while using same amount of catalase to observe results and patterns. Graph one particular shows the increased decomposition of H202 that was produced if the amount of catalase was increased by 2 hard disk drives every time. The more catalase that was added, the more quickly the decomposition was. The fastest and the most efficient decomposition was once there were 12 catalase filtering papers in 10mL of H202. The graduated cylinder was stuffed with oxygen in 1 minute and 46 seconds. Graph 2 shows that the decomposition rate slept relatively comparable with two catalase hard disk drives and different, elevating amounts of H202. This...